مرکز تحقیقات شیمی دارویی و گیاهی انگلیسی

High performance liquid chromatography- mass spectrometer (LC-MS)

The high performance liquid chromatography- mass spectrometer (LC-MS) is used to identify and quantify natural products constituents in plants extracts and other complex organic mixtures. Our center is equipped with a Shimadzu LCMS-2010EV. The Analyzer is Q-array Octapole quadropoe ( QoQ system) mass spectrometer. The MS is coupled to a LC-20AD HPLC by a number of interfaces, including electrospray ionization (ESI), and atmospheric pressure chemical ionization (APCI) respectively. The HPLC is also coupled to diode array detector (DAD) by which recording of the UV spectrum of each components is possible.

Combination of the ultra performance liquid chromatography (UPLC) with the ESI or APCI mass spectrometry and DAD enables us to analyze a wide range of organic substances. We use the LC-MS for analyzing non-volatiles, either synthetics or natural products for example antioxidant polyphenols , sugars, flavonoids , alkaloids, oxygenated and glycosylated terpenes etc.